Oregon National Primate Research Center
Oregon Health and Science University

The dataset includes resting state and structural data from 2 monkeys.

Usage Agreement

Creative Commons – Attribution-NonCommercial Share Alike (CC-BY-NC-SA)- Standard INDI data sharing policy. Prohibits use of the data for commercial purposes.

Species

Macaca mulatta

Sample Description

• Sample size: 2
• Age distribution: 5 years
• Weight distribution: 7.3-8.5kg
• Sex distribution: Both male

Phenotypic Assessments

Scan Procedures and Parameters

Ethics approval: The animal procedures were conducted in accordance with National Institutes of Health guidelines on the ethical use of animals and were approved by the Oregon National Primate Research Center (ONPRC) Institutional Animal Care and Use Committee.

Animal care and housing: Animals were indoor housed with pre-established male partners of similar age. Pairs were separated for fasting the night before a scan and reunited once fully awake post scan. Animal husbandry and care is maintained by ONPRC Department of Comparative Medicine clinical and behavioral support was provided by the Clinical Services Unit and the Behavioral Services Unit respectively.

Any applicable training: None

Scanning preparations

Anesthesia procedures: After an overnight fast, the animals were sedated by IM injection with 10mg/kg of ketamine for removal from cage and transport, intubation and catheter placement. Once intubated the sedation level was maintained with 1-1.5% isoflurane. At this time 8-12 mls of blood was drawn from the femoral vein for blood iron level monitoring and blood banking. Rectal temperature was taken pre and post scan.

Time between anesthesia and scanning: Time between initial ketamine sedation and placement in the coil averaged 20 mins. The BOLD session of the scan was started at a minimum of 45 minutes post ketamine injection.

Head fixation: Head was supported in coil with the use of foam padding and wedges.

Position in scanner and procedure used: Animals were placed in dorsal recumbency with head centered in the knee coil.

Contrast agent: Ferumoxytol at 8 mg/kg IV

During scanning

Physiological monitoring: Animals were continuously monitored remotely for SpO₂, respiratory rate, ETCO₂, pulse and blood pressure throughout the scan with measurements being recorded every 10-15 mins.

Additional procedures: Body temperature was maintained with a recirculating water mat and room temperature regulation. Catheter was kept patent with the remote administration of .5-1 mL of saline solution IV at regular intervals.

Scan sequences

• Scanner type: 3 Tesla Siemens Tim Trio
• Head coil: 15 channel knee coil
• Optimization of the magnetic field prior to data acquisition:
• Resting-state:
  • Voxel resolution: 1.5 x 1.5 x 1.5mm
  • TE: 25ms
  • TR: 2070ms
  • Flip angle: 90°
• Structural:
  • T1
  • MPRAGE Sequence
  • Voxel resolution: 0.5 x 0.5 x 0.5 mm
  • TE: 3.33ms
  • TR: 2600ms
  • TI: 900ms
  • Flip angle: 8°
  • T2
  • Voxel resolution: 0.5 x 0.5 x 0.5 mm
  • TE: 407ms
  • TR: 3200ms

Personnel

• Elinor L. Sullivan^1,2
• Julian Ramirez³
• Eric Feczko³
• Jennifer Bagley¹
• Eric Earl³
• Oscar Miranda-Domingue³
• Damien Fair³

¹Divisions of Neuroscience and Cardiometabolic Health, Oregon National Primate Research Center, Beaverton, OR
²Department of Human Physiology, University of Oregon, Eugene, OR
³Department of Behavior Neuroscience, Department of Psychiatry, Advanced Imaging Research Center, Oregon Health and Science University, Portland, OR

Acknowledgements

We appreciate assistance provided by Advanced Imaging Research Center technicians Michael Reusz and Vincent Warren.

Funding

This publication was supported by grant number R01 MH107508R01 (ES) from the National Institute of Mental Health and grant number P51 OD011092 for the operation of ONPRC.

Downloads

Click here to download the data. Users will first be prompted to log on to NITRC and will need to register with the 1000 Functional Connectomes Project website on NITRC to gain access to the PRIME-DE datasets.